Vitrification of Crabapple, Pear, and Geum on Gellan Gum-solidified Culture Medium
نویسندگان
چکیده
منابع مشابه
High rate of N2 fixation by East Siberian cryophilic soil bacteria as determined by measuring acetylene reduction in nitrogen-poor medium solidified with gellan gum.
For evaluating N(2) fixation of diazotrophic bacteria, nitrogen-poor liquid media supplemented with at least 0.5% sugar and 0.2% agar are widely used for acetylene reduction assays. In such a soft gel medium, however, many N(2)-fixing soil bacteria generally show only trace acetylene reduction activity. Here, we report that use of a N(2) fixation medium solidified with gellan gum instead of aga...
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Gellan gum is a hydrogel-forming polysaccharide when combined with monovalent or divalent cations such as sodium, magnesium, potassium or calcium. Commercially, gellan gums are sold with trace amounts of these cations, which have been proven to affect the gelation and mechanical properties of the resultant hydrogels. A new method based on impedance analysis for determining the gel transition te...
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Successful culturing of tissues within polysaccharide hydrogels is reliant upon specific mechanical properties. Namely, the stiffness and elasticity of the gel have been shown to have a profound effect on cell behaviour in 3D cell cultures and correctly tuning these mechanical properties is critical to the success of culture. The usual way of tuning mechanical properties of a hydrogel to suit t...
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ژورنال
عنوان ژورنال: HortScience
سال: 1990
ISSN: 0018-5345
DOI: 10.21273/hortsci.25.12.1648